Prof. Mehmet Haberal is presented with The Award of The Spanish Order of The Civil Merit (Cruz de Official) by His Majesty King Felipe VI, King of Spain.
We are honored to announce that Prof. Dr. Seza Özen who is the member of Honorary Advisory Board of JBACHS has already won the Aziz Sancar Science Award of TUSEB.
We are happy to announce that The Journal of Basic and Clinical Health Sciences (JBACHS) is indexed by the Emerging Sources Citation Index since November 2017, and indexed by the Ulakbim-TR since 2017.
Journal of Basic and Clinical Health Sciences 2020 , Vol 4 , Issue 2
Xenograft Tumor Volume Measurement in Nude Mice: Estimation of 3D Ultrasound Volume Measurements Based on Manual Caliper Measurements
Mustafa Mahmut Baris1,Efe Serinan2,Meryem Calisir3,Kursat Simsek1,Safiye Aktas2,Osman Yilmaz3,Sevgi Kilic Ozdemir4,Mustafa Secil1
1Dokuz Eylul University School of Medicine, Radiology, Izmir, Turkey
2Dokuz Eylul University Institute of Oncology, Basic Oncology, Izmir, Turkey
3Dokuz Eylul University, Laboratory of Animal Science, Izmir, Turkey
4Izmir Institute of Technology, Chemical Engineering, Izmir, Turkey
DOI : 10.30621/jbachs.2020.902 Objectives: Volume measurement of subcutaneous xenograft tumors in nude mice models is an important metric to assess tumor growth or response to therapy. Manual calipers are widely used for this purpose. But the measurements with manual calipers may be inaccurate. Contrarily, three-dimensional (3D) ultrasonographic measurements give reliable and accurate tumor volume calculation. We aim to; evaluate the accuracy of common four formulas given in the literature to estimate xenograft tumor volumes based on manual caliper measurements and offer a new coefficient for a better estimation of the tumor volumes.

Patients and Methods: Detailed manual diameter measurements of xenograft tumors were in 14 nude mice performed using Vernier caliper. Tumor volumes were calculated using the suggested formulas in the literature based on manual measurements. 3D ultrasound volume measurements were performed on same xenograft tumors using high resolution Vevo 2100 imaging system. To propose a new coefficient; means of ratio between manual and ultrasound volume measurement values were used. Also, data set was divided into two subgroups as tumor volume under 800 mm3 and over 800 mm3. New coefficients for each subgroup were defined.

Results: Only with prolate ellipsoid formula there was no statistically significant difference between volume measurements with two methods (p=0,24). Our proposed formula (0,45 L*W*H) could estimate tumor volumes as good as prolate ellipsoid formula. Coefficient 0,35 and 0,81 in the same formula were found efficient in the selected subgroups.

Conclusion: Using one common coefficient/formula for tumor volume estimation in any tumor size can be inaccurate. Appropriate coefficient should be chosen according to the dataset worked with. Keywords : Tumor volume, nude mice, ultrasonography, 3-D imaging, laboratory animal science