Prof. Mehmet Haberal is presented with The Award of The Spanish Order of The Civil Merit (Cruz de Official) by His Majesty King Felipe VI, King of Spain.
We are honored to announce that Prof. Dr. Seza Özen who is the member of Honorary Advisory Board of JBACHS has already won the Aziz Sancar Science Award of TUSEB.
We are happy to announce that The Journal of Basic and Clinical Health Sciences (JBACHS) is indexed by the Emerging Sources Citation Index since November 2017, and indexed by the Ulakbim-TR since 2017.
Journal of Basic and Clinical Health Sciences 2020 , Vol 4 , Issue 2
1,25-Dihydroxyvitamin D3 Induces N-Myc Downstream Regulated Gene-2 Expression In Papillary Thyroid Carcinoma Cells
Murat Sipahi1,Didem Keleş Bartık2,Mehmet Doruk3,Fırat Bayraktar4,Gülgün Oktay5
1Dokuz Eylül University, Institue of Health Sciences, Department of Biochemistry, İzmir, Turkey
2İzmir University of Economics, Vocational School of Health Services, Department of Medical Laboratory Techniques, İzmir, Turkey
3İzmir Bozyaka Training and Research Hospital, Division of Endocrinology and Metabolism, Department of Internal Medicine, İzmir, Turkey
4Dokuz Eylül University, Faculty of Medicine, Internal Medicine, Department of Internal Medicine Endocrinology And Metabolic Diseases, İzmir, Turkey
5Dokuz Eylül University, School of Medicine, Department of Medical Biochemistry, İzmir, Turkey
DOI : 10.30621/jbachs.2020.1012 Purpose: In addition to its role in serum calcium homeostasis, the anti-tumor function of 1,25-dihydroxyvitamin D3 (calcitriol) in cancer development is well established. N-myc Downstream Regulated Gene 2 which functions as a tumor suppressor gene has recently been shown to be downregulated in various cancer leading to increased tumor incidence, progression and metastasis. The goal of this study was to investigate the possible effects of calcitriol treatment on NDRG2 expression in BCPAP papillary thyroid carcinoma cells.

Methods: The experiments were carried on human primary thyroid follicular epithelial cells (Nthy-ori-3-1), and human papillary thyroid carcinoma cells (BCPAP). The half maximal inhibitory concentration (IC50) of calcitriol on BCPAP cells was determined by WST-1 assay. BCPAP cells were treated with 15 and 30μM calcitriol for 24, 48, and 72 hours, respectively. Basal NDGR2 expression in Nthy-ori-3–1 and BCPAP cells as well as the alterations on NDRG2 expression in calcitriol treated BCPAP cells were evaluated with western blot.

Results: A significant downregulation of NDRG2 was observed in BCPAP cells when compared to Nthy-ori-3–1 cells (p<0.01). IC50 dose of calcitriol was found to be 64, 54 and 43μM for 24, 48 and 72 hours, respectively. NDRG2 protein expression levels were significantly increased in 30μM calcitriol treated BCPAP cells after 48 hours (p<0.05).

Conclusions: Calcitriol induced NDRG2 protein expression in BCPAP cells. We predict that calcitriol increased NDRG2 protein levels in BCPAP cells via c-Myc repression, which is upregulated by aberrant Wnt/β-catenin signaling. Further investigation is required to enlighten the possible effect mechanisms of calcitriol in BCPAP cells. Keywords : 1,25-Dihydroxyvitamin D3, thyroid cancer, papillary thyroid cancer, N-myc downstream regulated gene 2