Prof. Mehmet Haberal is presented with The Award of The Spanish Order of The Civil Merit (Cruz de Official) by His Majesty King Felipe VI, King of Spain.
We are honored to announce that Prof. Dr. Seza Özen who is the member of Honorary Advisory Board of JBACHS has already won the Aziz Sancar Science Award of TUSEB.
We are happy to announce that The Journal of Basic and Clinical Health Sciences (JBACHS) is indexed by the Emerging Sources Citation Index since November 2017, and indexed by the Ulakbim-TR since 2017.
Journal of Basic and Clinical Health Sciences 2020 , Vol 4 , Issue 3
Assessment of Rac1 and β-PAK Expressions in a Mouse Model for Contrast-Induced Nephropathy
Yasar Aysun Manisaligil1,Serap Cilaker Micili3,Servet Kizildag5,Aslı Celik6,Husnu Alper Bagriyanik3,Mukaddes Gumustekin2
1Dokuz Eylul University, Vocational School of Health Services, Medical Imaging Techniques Program, Izmir, Turkey
2Dokuz Eylul University, Faculty of Medicine, Department of Molecular Medicine, Izmir, Turkey
3Dokuz Eylul University, Faculty of Medicine, Department of Histology and Embrylogy, Izmir, Turkey
4Dokuz Eylul University, Institute of Health Sciences, Department of Histology and Embrylogy, Izmir, Turkey
5Dokuz Eylul University,Vocational School of Health Services, Medical Laboratory Techniques Program, Izmir, Turkey
6Dokuz Eylul University, Faculty of Medicine, Department of Laboratory Animal Science,, Izmir, Turkey
7Izmir International Biomedicine and Genome Intitute, (iBG-izmir), Izmir, Turkey
8Dokuz Eylul University, Faculty of Medicine, Department of Pharmacology, Izmir, Turkey
DOI : 10.30621/jbachs.2020.1057 Purpose: Radiocontrast-induced nephropathy is an important clinical problem in high-risk patients. The mechanism of cytotoxic effect of radiocontrast media on kidneys is still not fully clarified. Rac1/β-PAK pathway has been shown to play a role in other nephropathies such as diabetic nephropathy. In this study, it was aimed to determine whether Rac1/β-PAK signalling pathway has any role in the development of contrast-induced nephropathy (CIN).

Methods: Adult male Balb/C mice were used. A single dose of iohexol was given intraperitoneally. Thirty-two mice were divided into 5 groups including control (Group1, n=4), pretreatment (Group 2, n=7), low dose (2 g iodine/kg) iohexol (Group 3, n=7), medium dose (2.5 g iodine/kg) iohexol (Group 4, n=7) and high dose (3 g iodine/kg) iohexol (Group 5, n=7). The animals were sacrificed 24 hours after iohexol administration. Nephropathy were evaluated by histological and biochemical analysis methods. Expressions of Rac1 and β-PAK were evaluated by immunohistochemical method. Apoptosis was assessed by TUNEL method.

Results: It was found that severity of nephropathy, apoptosis and β-PAK expressions significantly increased as iohexol dosage increased. Although Rac1 expression was higher in the iohexol group in comparison to the control and pretreatment groups, its increase did not show a dose-dependent manner. There was no significant difference among the groups in terms of serum creatinine. Serum cystatin C were increased in all groups compared to the control group, but significant increase was observed only in the pretreatment group.

Conclusions: Our findings suggest that the Rac1/β-PAK signal transduction pathway may have a role in the contrast-induced nephropathy model. Keywords : apoptosis, contrast-induced nephropathy, iohexol, mouse, Rac1