Prof. Mehmet Haberal is presented with The Award of The Spanish Order of The Civil Merit (Cruz de Official) by His Majesty King Felipe VI, King of Spain.
We are honored to announce that Prof. Dr. Seza Özen who is the member of Honorary Advisory Board of JBACHS has already won the Aziz Sancar Science Award of TUSEB.
We are happy to announce that The Journal of Basic and Clinical Health Sciences (JBACHS) is indexed by the Emerging Sources Citation Index since November 2017, and indexed by the Ulakbim-TR since 2017.
Journal of Basic and Clinical Health Sciences 2020 , Vol 4 , Issue 3
Berberine Attenuates Cerebral Vasospasm After Experimental Subarachnoid Hemorrhage Via Modulating AMPK/Rho Pathway
Ozge Altintas Kadirhan1,Meltem Kumas2,Ersin Karatas3,Hasan Serdar Mutlu4,Abdurrahim Kocyigit3,Talip Asil5
1Beykent University, Faculty of Medicine, Department of Neurology, İstanbul, Turkey
2Dokuz Eylul University, Faculty of Veterinary Medicine, Department of Histology and Embryology, Izmir, Turkey
3Bezmialem Vakıf University, Facult of Medicine, Department of Medical Biochemistry, istanbul, Turkey
4Bezmialem Vakıf University, Facult of Medicine, Department of Medical Biochemistry, istanbul, Turkey
5Bezmialem Vakıf University, Facult of Medicine, Department of Medical Biochemistry, istanbul, Turkey
DOI : 10.30621/jbachs.2020.1125 Purpose: Our goal is to clarify the effectiveness of berberine (BBR) on cerebral vasospasm induced by subarachnoid hemorrhage.

Methods: Thirty male Sprague-Dawley rats (350–400 g) were randomly allocated to five groups (sham group, SAH, BBR, SAH+BBR1 or SAH+BBR2. Experimental SAH model was induced by applying autologous blood into the cisterna magna at interval of 48 hours. To evaluate early and late effects of BBR, we allocated BBR treated groups as SAH+BBR1 and SAH+BBR2 (respectively, received BBR at a dose of 20 mg/kg 15 minutes and 6 hours after first SAH induction). Rats were sacrificed on 72-hour after the study onset. Cross-sections of basilar artery was investigated by histologically. Total antioxidant status (TAS) and total oxidant status (TOS) of brain tissue were studied by spectrophotometric assay. Oxidative stress index (OSI) was calculated. NAPPH Oxidase 4 (NOX4) enzyme levels were measured by ELISA method. Endothelial nitric oxide synthase (e-NOS), phosphorylated e-NOS (pe-NOS), AMP-activated protein kinase (AMPK), phosphorylated AMPK (pAMPK), Rho kinase and cingulin protein expressions were detected by Western blot analysis.

Results: SAH+BBR1 and SAH+BBR2 groups significantly demonstrated lower OSI values, increased basilar artery cross-sectional luminal area in comparison with the SAH group. Increased Phosho-eNOS, eNOS, P-AMPK levels and Cingulin expression, decreased Nox4 and Rho-kinase levels were shown in BRB treated SAH groups relative to the SAH group.

Conclusion: Berberine might be a neuroprotective agent to improve impaired cerebrovascular spasm. Keywords : berberine, subarachnoid hemorrhage, cerebral vasospasm, AMP-Activated Protein Kinase, Rho kinase, endothelial dysfunction